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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/03/2016 |
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Data da última atualização: |
04/03/2016 |
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Autoria: |
LEMOS, V. F.; GUARANÁ, E. L. S.; AFONSO, J. A. B.; FAGLIARI, J. J.; SILVA, P. C.; SOARES P. C.; MENDONÇA, C. L. de. |
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Afiliação: |
VÂNIA F. LEMOS, UFRPE; EDUARDO L. S. GUARANÁ, UFRPE; JOSÉ A. B. AFONSO, UFRPE; JOSÉ J. FAGLIARI, UNESP; PAULO C. SILVA, UNESP; PIERRE C. SOARES, UFRPE; CARLA LOPES DE MENDONÇA, UFRPE. |
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Título: |
Effects of intramammary infection on whey proteinograms os sheep during lactation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 3, p. 230-236, mar. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep. MenosThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for ... Mostrar Tudo |
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Palavras-Chave: |
Marcador biológico; Mastite subclínica; Proteína de fase aguda; Subclinical mastitis. |
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Thesagro: |
Leite; Ovelha. |
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Thesaurus Nal: |
Acute phase proteins; Biomarkers; Ewes; Milk. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140638/1/Effects-of-intramammary-infection.pdf
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Marc: |
LEADER 02780naa a2200313 a 4500
001 2039647
005 2016-03-04
008 2015 bl uuuu u00u1 u #d
100 1 $aLEMOS, V. F.
245 $aEffects of intramammary infection on whey proteinograms os sheep during lactation.
260 $c2015
520 $aThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep.
650 $aAcute phase proteins
650 $aBiomarkers
650 $aEwes
650 $aMilk
650 $aLeite
650 $aOvelha
653 $aMarcador biológico
653 $aMastite subclínica
653 $aProteína de fase aguda
653 $aSubclinical mastitis
700 1 $aGUARANÁ, E. L. S.
700 1 $aAFONSO, J. A. B.
700 1 $aFAGLIARI, J. J.
700 1 $aSILVA, P. C.
700 1 $aSOARES P. C.
700 1 $aMENDONÇA, C. L. de
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 3, p. 230-236, mar. 2015.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 13 | |
| 2. |  | JULIANO, R. S.; FAGLIARI, J. J.; SILVA, L. A. F.; FIORAVANTI, M. C. S. Proteinograma sérico de bovinos da raça Curraleiro obtido por eletroforese em gel de poliacrilamida. (Serum protein concentration in Curraleiro cattle determined by sodium dodecyl sulphate-polyacrilamide gel.) In: CONGRESSO BRASILEIRO DE BUIATRIA, 7., 2007, Curitiba, [Resumo expandido] Curitiba, PR, 2007. 1 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pantanal. |
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| 4. | | ROCHA, T. G.; BORTOLETTO, C.; SILVA, D. G.; SIMPLÍCIO, K. M. M. G.; ZAFALON, L. F.; FAGLIARI, J. J. Serum proteinogram and biochemistry of Holstein cows in the peripartum period. Pesquisa Agropecuária Brasileira, v.39, n.5, maio, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 5. | | ROCHA, T. G.; SILVA, K. R. da; BORTOLETTO, C.; MONTASSIER, H. J.; BUZINARO, M. G.; ZAFALON, L. F.; ALFIERI, A. A.; FAGLIARI, J. J. Passive immunity transfer from holstein cows immunized or not against rotavirus to its calves. Biológico, São Paulo v. 77, (supl. 2), p. 44, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. | | LEMOS, V. F.; GUARANÁ, E. L. S.; AFONSO, J. A. B.; FAGLIARI, J. J.; SILVA, P. C.; SOARES P. C.; MENDONÇA, C. L. de. Effects of intramammary infection on whey proteinograms os sheep during lactation. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 3, p. 230-236, mar. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | ROCHA, T. G.; FLORENTINO, F. D.; BORTOLETTO, C.; SILVA, D. G. da; BUZINARO, M. da G.; ZAFALON, L. F.; FAGLIARI, J. J. Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, MG, v. 68, n. 4, p. 865-872, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | ROCHA, T. G.; NOCITI, R. P.; BORTOLETTO, C.; SANTOS, R. A. L. dos; SANTANA, R. C. M.; JORGE, R. L. N.; NOGUEIRA, C. A. da S.; FAGLIARI, J. J. Bioquímica sérica de vacas da Raça Holandesa no período pré-parto. In: CONGRESSO BRASILEIRO DE BUIATRIA, 10.; SEMANA DO MÉDICO VETERINÁRIO DO PARÁ, 37.; SIMPÓSIO PARAENSE DE MEDICINA VETERINÁRIA, 5., 2013, Belém. Anais... Belém: Associação Brasileira de Buiatria, 2013. 4p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | ROCHA, T. G.; NOCITI, R. P.; SANTOS, R. A. L.; BORTOLETTO, C.; NOGUEIRA, C. A. S.; JORGE, R. L. N.; SANTANA, R. C. M.; ZAFALON, L. F.; SILVA, F. D. F.; FAGLIARI, J. J. Influence of parturition on biochemical analysis of milk whey from holstein cows in the colostral period. In: WORLD VETERINARY CONGRESS, 150., 2013, Prague, Czech Republic. Proceedings... Prague: World Veterinary Association, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | ROCHA, T. G.; NOCITI, R. P.; BORTOLETTO, C.; SANTOS, R. A. L. dos; NOGUEIRA, C. A. da S.; JORGE, R. L. N.; SANTANA, R. C. M.; ZAFALON, L. F.; FAGLIARI, J. J. Influência do número de parições sobre análises bioquímicas do soro lácteo de vacas holandesas durante a fase colostral. In: CONGRESSO BRASILEIRO DE BUIATRIA, 10.; SEMANA DO MÉDICO VETERINÁRIO DO PARÁ, 37.; SIMPÓSIO PARAENSE DE MEDICINA VETERINÁRIA, 5., 2013, Belém. Anais... Belém: Associação Brasileira de Buiatria, 2013. 4p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | ROCHA, T. G.; SILVA, K. R.; SILVA, F. D. F.; ALFERI, A. A.; SILVA, D. G.; MONTASSIER, H. J.; BUZINARO, M. da G.; GREGORI, F.; ZAFALON, L. F.; FAGLIARI, J. J. Evaluation of passive immunity transfer against G6P[1] rotavirus in Holstein Calves by ELISA. Pakistan Veterinary Journal, mar. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 12. | | ROCHA, T. G.; BORTOLETTO, C.; SILVA, D. G.; SIMPLÍCIO, K. M. M. G.; SILVA, P. C.; SILVA, F. D. F.; BUZINARO, M. G.; ZAFALON, L. F.; FAGLIARI, J. J. Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea. O Biológico: São Paulo, v.77, suplemento 2, p.60, 2015. 77| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 13. | | ROCHA, T. G.; NOCITI, R. P.; SANTOS, R. A. L. dos; BORTOLETTO, C.; SANTANA, R. C. M.; NOGUEIRA, C. A. da S.; JORGE, R. L. N. JORGE; FAGLIARI, J. J. Teor sérico de minerais de vacas da raça holandesa no período pré-parto. In: CONGRESSO BRASILEIRO DE BUIATRIA, 10.; SEMANA DO MÉDICO VETERINÁRIO DO PARÁ, 37.; SIMPÓSIO PARAENSE DE MEDICINA VETERINÁRIA, 5., 2013, Belém. Anais... Belém: Associação Brasileira de Buiatria, 2013| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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